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| Registros recuperados: 269 | |
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| Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent. |
| Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at... |
| Tipo: Text |
Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf |
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| Silva,Cássio Prinholato da; Costa,Tássia R.; Paiva,Raquel M. Alves; Cintra,Adélia C. O.; Menaldo,Danilo L.; Antunes,Lusânia M. Greggi; Sampaio,Suely V.. |
| Abstract Background Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. Methods The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops jararacussu; BthTX-I; Antitumor potential; Apoptosis; Cell cycle alterations. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348 |
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| Lopez,Lidia M.; Dubin,Marta; Carrizo,Patricia H.; Burgos,Mario H.; Pellegrino de Iraldi,Amanda; Stoppani,Andrés O. M.. |
| CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a b-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; O-naphthoquinones; Hepatocytes; Chromatin Alteration; Mitochondrial Swelling Membrane; Blebs. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200007 |
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| Alihemmati,Alireza; Yousefi,Hadi; Ahmadiasl,Naser; Habibi,Parisa. |
| ABSTRACT Ischemia-reperfusion injury was seen in strokes, myocardial infarctions, acute kidney injury, mesenteric ischemia, liver and systemic shock. Renal ischemia-reperfusion is more importance in the setting of kidney transplantation that affects distant organs. In this study forty Male Albino Wistar rats (200-250g) were randomly divided in four group (n=10) including control, sham operation group, nephrectomy and IRI group. All rats anesthetized with intraperitoneal injection of ketamine (50 mg/kg) and xylazine (10 mg/kg) and maintained the core body temperature at approximately 37°C. For inducing IRI group, it was performed right nephrectomy, and in continuing, the left kidney pedicle occluded to 45 min via nontraumatic microvascular clamp for making... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Renal Ischemia-reperfusion injury; Glycogen accumulation; Apoptosis; Heart; Rat. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100443 |
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| Schinoni,Maria Isabel; Paraná,Raymundo; Cavalcante,Daniel. |
| Hepatitis C is a worldwide endemic disease, affecting roughly 200 million people. It has a variable prognosis, depending on the progression to fibrosis. During the last five years, the importance of apoptosis for the pathogenesis of various diseases, including hepatitis, has been recognized. It has been suggested that an increase in T cell-apoptosis during a hepatitis C virus infection is the cause of impaired regulation of the immune cellular response, helping to maintain infection. Thus, the interest in discovering the probable mechanisms by which the hepatitis C virus perpetuates in the liver, and to determine the conditions that predispose for progression of this disease, makes investigation of apoptosis in hepatic injury of great interest. We have... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Hepatitis C; Liver fibrosis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200009 |
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| López-Herrera,A.; Ruiz-Sáenz,J.; Góez,Y.P.; Zapata,W.; Velilla,P.A.; Arango,A.E.; Urcuqui-Inchima,S.. |
| To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To elucidate the mechanisms responsible for this diversity, we searched for a possible relationship... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vesicular stomatitis virus; Apoptosis; Resistance; Bovine fibroblasts (Source: NLM). |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200007 |
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| Segovia,Y. |
| La apoptosis o muerte celular programada es un proceso que ocurre durante el desarrollo del sistema nervioso. El objetivo de este estudio fue observar los patrones de apoptosis que se producen durante el desarrollo embrionario de la retina, desde el estadio S16 al momento del nacimiento, mediante miscoscopía óptica y electrónica. Se utilizaron retinas de embriones de tortuga. Nuestros datos muestran que los primeros signos de apoptosis comienzan en el estadio S16, en la capa nuclear interna y alcanzan su máxima densidad tanto en la capa nuclear interna como en la capa de células ganglionares en S20, para extinguirse, prácticamente, en el momento del nacimiento. Por otra parte, la apoptosis sigue un gradiente centro-periferia. |
| Tipo: Journal article |
Palavras-chave: Trachemys scripta elegans; Apoptosis; Retina; Tortuga. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100020 |
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| da Silva,Jairo Pinheiro; de Carvalho,Camila Albuquerque Mello; Romualdo,Andressa Rodrigues; Schiavoni,Vagner Sarraipo; Barbosa,Marcello Henrique Nogueira; de Moraes,Marcella Suélen Torrecilla; Tirapelli,Daniela Petri Cunha; Fazan,Valéria de Paula Sassoli; Thomazini,José Antônio; Tirapelli,Luis Fernando. |
| The aim of this study is evaluate the efficacy of 904 nm laser diode in bone regeneration in the bone defect in diabetic rats. Six groups of 10 male Wistar rats and 2 mm bone defects drilled on the left and right tibia were used. The diabetic animals were treated with streptozotocin (40 mg/kg, i.v.). We compared the diode laser doses of treatment of bone defects 50 w 4 J/cm and 100 w 4J/. The right tibia was used for immunohistochemical analysis with the apoptosis markers XIAP and Caspase-3 and the left tibia was submitted to computer tomography (CT). Caspase-3 marker showed greater amount of apoptosis in all the untreated groups compared to both laser treatments. There was no statistical significance for XIAP marker. CT scan showed improvement of bone... |
| Tipo: Journal article |
Palavras-chave: Bone defect; Diabetes mellitus; Laser therapy; Regeneration; Apoptosis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100029 |
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| Davatz,Giovanna Castilho; Andreo,Jesus Carlos; Rodrigues,Antonio de Castro; Rosa Júnior,Geraldo Marco; Moraes,Luiz Henrique Rapucci. |
| The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated... |
| Tipo: Journal article |
Palavras-chave: Skeletal muscle; Denervation; Apoptosis; Rat. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300009 |
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| Amorim,Aline Teixeira; Marques,Lucas Miranda; Santos,Angelita Maria Oliveira Gusmão; Martins,Hellen Braga; Barbosa,Maysa Santos; Rezende,Izadora Souza; Andrade,Ewerton Ferraz; Campos,Guilherme Barreto; Lobão,Tássia Neves; Cortez,Beatriz Araujo; Monezi,Telma Alvez; Machado-Santelli,Glaucia Maria; Timenetsky,Jorge. |
| BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and... |
| Tipo: Journal article |
Palavras-chave: Ureaplasma diversum; Invasion; Apoptosis; HEp-2 cells. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038 |
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| Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L.. |
| Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013 |
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| Cruzans,P.R.; Carou,M.C.; Lorenzo,M.S.; Lombardo,D.M. |
| Se han identificado receptores de la hormona liberadora de gonadotrofina (GnRHR) en células de granulosa ovárica (CG) y en células luteales (CL). Asimismo, se ha observado que la activación de los GnRHR en CG regula la función gonadal. Estudios realizados utilizando agonistas de GnRH (GnRHa) en ovarios de ratas, in vivo e in vitro, demostraron un incremento de la atresia folicular. Nuestro objetivo fue estudiar la regulación de la apoptosis en una línea establecida de células de granulosa de ovario bovino (BGC-1) utilizando el acetato de leuprolide (LA) como GnRHa y el ANTIDE como antagonista competitivo. Nuestros resultados demuestran un aumento significativo de la proporción de células apoptóticas inducidas durante 24 y 48 h con 100 nM LA, confirmado... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; BGC-1; Acetato de leuprolide; GnRH. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982012000100011 |
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| Wang,Tao; Jiang,An -li; Zhang,Jian-ye; Jing,Fei. |
| To observe effect of E2F decoy DNA on proliferation and apoptosis of androgen-independent prostate cancer cell line PC-3M the binding specificity of the E2F decoy DNA to the PC-3M nuclear protein was detected by electrophoretic mobility shift assay (EMSA). E2F decoy DNA, ARE decoy DNA and Control decoy DNA were respectively transfected into PC-3M cells with lipofectamine.Their influence on cell proliferative activity was detected by MTT assay.The cell apoptotic rate was determined by flow cytometric(FCM) analysis and chromosome DNA ladder was detected by DNA gel electrophoresis.The change of mRNA expression of C-myc and CyclinD1 were detected by RT-PCR.The change of mRNA expression of C-myc and CyclinD1 were detected by Western-blot. EMSA demonstrated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Decoy DNA; Transcription factors; PC-3M cells; Apoptosis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200011 |
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| Oliveira,A.L.R.. |
| The rate of axonal regeneration, after sciatic nerve lesion in adult C57BL/6J mice, is reduced when compared to other isogenic strains. It was observed that such low regeneration was not due just to a delay, since neuronal death was observed. Two general mechanisms of cell death, apoptosis and necrosis, may be involved. By using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technique, we demonstrated that a large number of sensory neurons, as well as satellite cells found in the dorsal root ganglia, were intensely labeled, thus indicating that apoptotic mechanisms were involved in the death process. Although almost no labeled neurons or satellite cells were observed one week after transection, a more than ten-fold increase in... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: TUNEL; Apoptosis; Axotomy; Neuronal death; Satellite cells; Isogenic mice. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300012 |
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| REYES,PAZ A; VARGAS,MACARENA F; GARCÍA,KATHERINE P; RUBILAR,PAULINA S; NAVARRETE,PATRICIA A; FUENTES,PAMELA M; RÍOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H; RUBIO,VÍCTOR H; HECKELS,JOHN; CHRISTODOULIDES,MYRON; CÁRDENAS,HUGO; VELASQUEZ,LUIS A. |
| Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of... |
| Tipo: Journal article |
Palavras-chave: Apoptosis; Fallopian tube; Neisseria gonorrhoeae; Salpingitis; TNF-alpha. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400006 |
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| Registros recuperados: 269 | |
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